Exact(3)
Small E-values of these methods would indicate high quality hit to EST sequences.
Thus, one can rely on the appearance of a consensus sequence as another indication that a given compound is a high quality hit, as has been seen many times in screens of biologically encoded libraries of peptides or aptamers.
All 454 reads with a high quality hit against the genome (at least 90% of the length of the 454 sequence was aligned by MEGABLAST) were mapped within the genome and their distribution established as for the complete genome.
Similar(57)
They offer a rich source of high quality hits against diverse target sequences and have been used for three main purposes: firstly, to identify and validate targets in phenotypic screens; secondly, to block protein interactions with nanomolar potency binding affinities; thirdly as a source of more efficient cell penetrating peptides for the delivery of a wide range of biologics.
For unknown junctions predicted by SAW, nearly 30% of these junctions had high quality hits to EST sequences (Figure 5).
The majority of known junctions had high quality hits to EST sequences, while the majority of unknown junctions had low quality hits to EST sequences.
Expressed sequences are compared to the human genome to find high quality hits, and are then aligned to it using a spliced alignment model that allows long gaps, for modeling introns.
BLAST searches were restricted to only high quality "hits" (e-value of ≤.0001).
Ideally, one should employ an intracellular assay which is the most physiological relevant assay to find high quality hits.
We therefore tested if extra high quality hits could be recovered for Helicos tSMS sequences, by increasing the maximum number of indels allowed to two.
Reads of VELVET contigs that resulted in high quality hits against the mitochondrial genome of R. balthica were assembled against the latter using Geneious Pro 5.4.2 (http://www.geneious.com).
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