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It provides a high quality expression profile that appears to allow identification of differentially expressed genes.
A pre-requisite for the success of this approach is the availability of a high quality expression signature.
We belief that de novo synthesis of RNA- and codon-optimized genes will become a standard process for recombinant human protein production, and will serve to improve and standardize any application relying on reproducible, efficient and high quality expression.
For example, at the time of array design we were able to identify 13 high quality expression microarray studies of prostate cancer aggressiveness (See Tables S1 and S2 for full reference list).
Here we describe the development of a robust and highly sensitive profiling assay capable of generating high quality expression profiles in degraded/FFPE samples at a whole-transcriptome level.
In samples where microarray/FISH testing failed, high quality expression data could not be obtained despite multiple attempts.
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Select approximations may be relaxed for more precise inference once higher quality expression data or reliable information from other sources becomes available.
To get to biological insights simply, quickly, and seamlessly, the complete mouse and rat transcriptome solutions include intuitive analysis software that transforms the high-quality expression data generated by the assays into biologically meaningful information in minutes.
From the 64 samples, high-quality expression data were obtained for 56 samples.
However, our analysis only considers the 4,271 genes where we had high-quality expression information across all studies.
After filtering for high-quality expression data, 57 samples and 13,776 array probes were retained for differential expression analysis.
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