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Additionally, the long contig assemblies by the other programs generate more high quality BLAST hits than those found for VELVET.
Further, using a high quality BLAST alignment alone for the assignment gives this approach the ability to also assign a gene that lies in a gap in the human genome sequence.
If the genome address assignment agreed with the BLAST result, then the EST was assigned to the gene, if they disagreed, only a high quality BLAST result allowed EST to gene assignment (>= 92% identity, >= 100 bp alignment).
Investigating them more closely, however, revealed a large fraction of short contigs, redundant contigs, and a lower number of high quality BLAST hits in comparison to the other two assemblers.
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BAC: bacterial artificial chromosome; BES: BAC end sequence; SBH: single BLAST hit; PBH: paired BLAST hit; hqPBH: high quality paired BLAST hit; lqPBH: low quality paired BLAST hit.
Coking of coal blends using high volatile coals with poor coking abilities to produce a high quality coke for blast furnace application can be achieved by compacting the coal blend prior to the carbonization process.
SY63 has not only the traits of high yield, acceptable grain quality, blast resistance and wide adaptability, but also is suitable for cultivation as a ratoon rice.
Our control of automatic assembly of coding sequences assured that only high quality coding sequences assembled from Blast alignments (i.e. 99% match or greater) were analyzed.
After high quality sequence reads were obtained, BLAST analysis was performed as in Bainbridge et al [ 2].
For 59 fragments, high quality sequences were obtained and blasted against protein databases.
The results, using all wheat and barley probesets from the "high quality" set linked by a reciprocal Blast hit, are shown in Figure 8A.
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