We have developed a publicly available NSF45K oligo array and employed five methods to demonstrate that it can be used to obtain high-quality, biologically relevant data.
We have carried out extensive validation of these data sets using RT-qPCR (Additional file 1: Figure S1) which indicates that both the microarray and HTS experiments are producing high-quality, biologically relevant information.
Validation of a subset of these genes by RT-qPCR (Additional file 4: Figure S2) indicates that our RNA-seq data is high quality and biologically relevant.
Thus, future widespread use of biofuels depends on developing new process technologies to produce high quality transportation fuels from biologically derived feedstocks, which avoid this key handicap.
The most common treatment systems seen across the world are membrane bioreactors, sequence batch reactors and biologically aerated filters which produce high quality effluent.
This allows for estimation of daily free, unbound and biologically active 17β-estradiol and progesterone, and for high quality estimates of full cycle ovarian hormone profiles (Jasisenska and Jasienski, 2008).
Thus, one can rely on the appearance of a consensus sequence as another indication that a given compound is a high quality hit, as has been seen many times in screens of biologically encoded libraries of peptides or aptamers.
With large collections of high quality public data currently available, we carried out a comparative analysis of current day leads with other biologically relevant datasets.
