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Studies were of generally high quality (additional file 2).
From these 69,011 SNPs, 96 were empirically tested using the Illumina BeadXpress genotyping platform, of which 82 were considered high quality (Additional File 3).
RIN values for our total RNA extractions ranged from 9.2 – 9.4, a measure of high quality (Additional file 2: Figure S1).
A standard extraction protocol with Trizol yielded degraded RNA (not shown), while RNA extracted by a CTAB-based method was of high quality (Additional file 1).
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It suggests that in addition to high quality CPR, additional intervention aiming at preserving mitochondrial function and rapid reductions of cerebral metabolism during CA, such as intra-arrest hypothermia and pharmacologically induced cerebral hibernation, may be the new strategies for neurological protection in CA and CPR.
The alignment process yielded an average efficiency of 56% for the high quality reads (Additional file 1: Table S1).
A data set of 36,976 SNPs at Minor Allele Frequency (MAF) ≥ 4 % was generated from 57,418 high quality tags (Additional file 2).
The list of candidate reference miRNAs identified using all measurements was similar to the list identified using high quality measurements (Additional file 1: Table S8 and S9).
However, all three RCTs, of which two were regarded high quality ("see Additional file 8") [ 49, 50], showed no beneficial effect of vitamin B12 administration on haemoglobin concentrations and MCV.
As our objective was to identify the major venom proteins, and since no reference genome was available, we decided to use the Sanger technology to produce long, high quality sequences (Additional file 1: Figure S1).
Under the well-watered condition, the 2-DE gel of resistant cultivar YJ-1 showed 542 high quality spots (Additional file 1), while 11 unique, 12 up-regulated, 6 down-regulated and 6 disappeared spots were induced by drought stress, 17 unique, 15 up-regulated, 5 down- regulated and 7 disappeared spots were induced by A. flavus infection under drought stress (Additional file 2).
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