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This functional category is represented by 66.5%, 50%, and 100% of orthologs belonging to the high purifying, weak purifying and the divergent ortholog set, respectively.
Cox1, cox2, atp6, cytb, cox3 and nd3 showed the lowest Ka/Ks values (below 0.1), suggesting that those genes are under high purifying selection.
We discovered that to better survive from the random deletion process, persistent genes, which are under inherent high purifying selection pressure, organized as clusters.
Within the high purifying group (Ka/Ks <0.5), 211 sequences were specifically involved in ATP binding activity, while 13.5% of transcripts were metabolically active transcripts.
Reflecting the signature of high purifying selection predominantly identified by the LRTs and BEB values, the conservation index indicates high rates of purifying selection across eutherian mammals (Fig. 2).
Probably because of the functional roles played in the respiratory chain by the mitochondrial genome, PCGs showed high purifying selection and low nucleotide divergence among the species investigated here.
Similar(51)
Protein A affinity purification and desalting were sufficient to obtain all scFv-Fc antibodies in very high purify of >98%.
The TAP tag was more recently developed in order to obtain high purified protein samples for mass spectrometry analysis of protein complexes [ 29].
Thus, higher purifying selection on cancer genes could be treated as a trademark of higher miRNA targets.
Thus, gene duplicates in unicellular organisms are subject to higher purifying selection than their counterparts in multicellular eukaryotes.
Overall our results reveal that the three ribosomal protein S13 genes in Malus are undergoing higher purifying selection than neutral evolution, suggesting that their functionality is maintained by purifying selection.
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