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It is important to note that despite an inability to assign individual protection factors to these three residues, the high protection factor assigned collectively implies that all three are protected to some degree individually (because any one of the residues, if unprotected, would dramatically lower the overall protection factor).
We advised our patient to wear appropriate clothing and use sun cream with a high protection factor.
These animal studies also demonstrate that the use of a sunscreen with a high protection factor significantly reduces the severity of skin reactions.
However, the high protection factor for K28 is consistent with the SS-NMR detection of a salt bridge between K28 and D23 in Aβ40 samples incubated under conditions conducive to the formation of homogeneous two-filament fibrils.
However, the relatively high protection factor of 7 for K28 is consistent with an amyloid folding nucleus for the Aβ40 monomer proposed by Lazo et al. Most fibrils exhibited semiregular variations in width from ∼4 to ∼8 nm, suggesting a ribbonlike structure.
The high protection factor for this residue may be due to the eight N-terminal residues not included in the 2LMO model, or to quaternary contacts in the three-filament 2LMP and 2LMQ models, where L17 and H13 are in close contact with V36 and V39/40, respectively.
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Nuclear Magnetic Resonance NMRR) experiments identified the residues involved in the hydrogen-bonded network and demonstrated that these self-assembled β-sheet-based fibers exhibit high protection factors that bear resemblance to amyloids.
Such high protection factors indicate that no significant local conformational fluctuation exists in most parts of the protein including ice binding surface.
However, their results for fibrillar Aβ40 also indicated a relatively high level of exchange for residues 35 40, contrary to the high protection factors observed in this study.
Accordingly, the amides of C310, L308 and G306 showed high protection factors in NMR deuterium exchange experiments, confirming their involvement in H-bonds (Fig 3B).
All protons that are postulated as serving as hydrogen bond donors were found to have high protection factors in H/D exchange experiments.
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