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Local contrast was computed to detect regions of high pixel intensity variations (i.e., regions of the image likely to contain cells).
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Fluorescence distribution images of C. elegans after exposure to ZnO-NPs showed high pixel intensities with increase in concentration and decrease in size of NPs.
This is because both result in the signal being digitized into fewer gray levels, although it should be noted that the effect is not exactly the same due to the loss of high pixel intensities when reducing the bit depth.
Edges will have higher pixel intensity values than those surrounding it.
The highest pixel intensity was recorded with exposure of 10 nm NPs and shows more even distribution of fluorescence.
Confocal imaging of DiSBAC-loaded cells also reported depolarization (higher pixel intensity) for OS and AD cells exposed to ouabain and high K+ (Fig. 2B).
Since candidate points represent the highest pixel intensity, using different brightness and contrast will not affect the procedure.
Along a concentric ring-shaped region of interest across the emerging phloem poles, the latter appeared as dark foci of GUS staining with higher pixel intensity.
After background subtraction, the intensity map for heterochromatin regions in single cells was directly calculated by normalizing pixel intensity in the GFP-HP1 channel with the highest pixel intensity in the image.
We then applied a noise removal filter to SHG images to define the boundary between foreground and background, and the lower threshold in the histogram was set to 10% of the highest pixel intensity value.
Low MR signals are represented as higher pixel intensities in the MR image (Movie S2).
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