Sentence examples for high performance beads from inspiring English sources

The first method was purification using Ni Sepharose high performance beads in a batch approach.

The biotinylated PCR products were immobilized onto Streptavidin Sepharose high performance beads.

Primers were supplied by IDT, Streptavidin Sepharose High Performance beads were from GE Healthcare Life Sciences (Little Chalfont, UK) and all other reagents and equipment were from Qiagen.

The purification procedure consisted of incubating the solubilized membranes with 5 ml of Ni Sepharose High Performance beads (GE Healthcare) for 1 2 h at 4°C.

Pyrosequencing was performed using the PyroMark annealing buffer (Qiagen) and PyroMark binding buffer (Qiagen), 3 μL Streptavidin Sepharose High Performance beads (GE Healthcare, Stockholm, Sweden) and 350 mM pyrosequencing primer on the PyroMark Q24 (Qiagen) according to manufacturer's instructions.

Biotinylated proteins were incubated for 1 h with equal volume of Streptavidin-Sepharose high performance beads (GE Healthcare, Piscataway, NJ, USA), purified by repeated precipitations in Binding buffer and eluted with 100 mM 2-mercaptoethanol.

Ni2+-NTA Sepharose high-performance beads were purchased from GE-Hamersham Biosciences (Pittsburgh, PA).

Forty-six micro-litres of PCR product were added to 38 μL of binding buffer (Qiagen®) and 2 μL streptavidin sepharose high-performance beads (GE Healthcare®).

Then, 22 μL of PCR product was added to 40 μL of binding buffer, 16 μL of ultrapure water and 2 μL of streptavidin sepharose high-performance beads.

A portion of 20  μl of biotinylated PCR product was immobilised on streptavidin-coated Sepharose High-Performance beads (Amersham Biosciences, Piscataway, NJ, USA) and processed to obtain a single-stranded DNA using the PSQ 96 Sample Preparation Kit (Biotage) according to the manufacturer's instructions.

Streptavidin sepharose high-performance bead (GE healthcare Bio-Sciences Corp., Piscataway, NJ, USA) purification was performed by incubating 2.5 μl PCR product with 3 μl beads and 25 μl binding buffer (3 mM Tris-HCl, pH 7.5, 0.3 mM ethylenediamine tetraacetic acid, 0.6 M NaCl) for 5 minutes under continuous agitation.