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Purified samples were placed into a 96-well plate holder or single vial holder of an Agilent 1200 Series HiP-ALS (High Performance Automated Liquid Sampler, G1367B) and sequentially injected by 0.8 ml/min flow from an Agilent 1200 Series Isocratic pump (G1310A).
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The instrument features high performance optics, automated liquid handling, and an environmental control center, all directed through an intuitive touchscreen interface.
We describe a fully automated high performance liquid chromatography 9.4 tesla Fourier transform ion resonance cyclotron (FTICR) mass spectrometer system designed for proteomics research.
SNPs for each exon, exon/intron boundaries, and 10 kb upstream (5′) to the AKT1 gene (Genbank NM_001014431.1) were identified by screening a panel of 96 ethnically diverse genomic DNA samples with denaturing high performance liquid chromatography and automated sequencing.
A1C was measured by a Tosah G7 automated high performance liquid chromatograph (in a laboratory with National Glycohemoglobin Standardization Program level I certification for traceability to the Diabetes Control and Complications Trial reference); FPG was measured enzymatically on an Hitachi 917 autoanalyzer.
Immunoaffinity purification, reversed phase high performance liquid chromatography (RP-HPLC), automated chemical sequencing and peptide synthesis have been used to identify and characterize octamer and nonamer self-peptides from a murine B cell lymphoma cell line.
HbA1c concentration was measured by ion exchange high performance liquid chromatography (Tosoh Automated Glycohemoglobin Analyser HLC-723 G7, Tosoh Bioscience, Minato, Japan), aligned to the Diabetes Control and Complication Trial standard.
The use of fully deformable registration methods, cohort atlases, and user-defined manual tracings are recommended for highest performance in fully automated hippocampus segmentation.
Described in this study is a fully automated two-dimensional high performance liquid chromatography (2D-HPLC) method for characterizing protein aggregation of crude in-process bioreactor samples.
Fraction of HbA1c was measured by a high performance liquid chromatography method (a Tosoh Automated Glycohaemoglobin Analyzer HLC-723 G. Reference interval: 0.042-0.063).
5HIAA concentrations were measured using automated solid phase extraction and high performance liquid chromatography (HPLC) as previously described (Zhao et al, 2001).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com