Exact(4)
Indeed, we found a peak of high pause frequency just before the start of TM2 (p = 3 × 10−6, 2-sample t test), extending from five codons before TM2 to the second codon of the TM.
Second, it is unclear to me how the kinetic parameters at the pointed end measured here for single filaments agree so well with previous bulk measurements, given the high pause probability illustrated in Figure 4.
This suggests that the microtubules shortened in MC+1 cells are either taking longer time to grow (high growth duration) or staying in a pause phase (high pause duration).
These results are now discussed throughout the main text and included in Figure 3 figure supplements 1-3 and Figure 5. Second, it is unclear to me how the kinetic parameters at the pointed end measured here for single filaments agree so well with previous bulk measurements, given the high pause probability illustrated in Figure 4.
Similar(56)
The authors also report high pausing probability in presence of NEM-myosin.
These results suggest that the genes whose expression is confirmed by RNAseq can be classified into three categories by combining ChIPseq data concerning Ser2P/Ser5P: 'High pausing, High elongation (Ser5P+, Ser2P+)', 'High pausing, Low elongation (Ser5P+, Ser2P- ', and 'Low pauSer2P- 'w elongandon (Ser5P-, Ser2P-)'.
The amount of mRNA expression of the genes grouped by phased phosphorylation of Ser2 and Ser5 of RNAPII had a tendency to increase with the level of phosphorylation, leading the predicted three category mode: 'High pausing, High elongation', 'High pausing, Low elongation', and 'Low pausing, Low elongation'.
The remaining 844 genes ('MBT-zygotic genes') frequently have high Pol II occupancy at the pausing site and a high pausing index, suggesting that Pol II pausing is widespread during the MBT.
Normally at higher pause time, nodes may stick on to the terrain boundaries for longer time making itself to be invisible for others.
This leads to path breaks for longer time causing a reduced amount of packet delivery ratio at higher pause times as shown in Figure 10.
Most of the significant correlations with total and sub-topic symptom scores seen with ratio of pause time were also correlated with average pause length; the only sub-topic that does not follow this rule is the HAMD measure of Early Morning Insomnia, which shows a higher pause ratio with worsening of condition.
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