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This high mutation frequency suggests that GNAQ mutational analysis can also be useful in the differential diagnosis of peripherally located melanotic schwannoma with blue nevus.
The high mutation frequency in both familial and sporadic patients makes LRRK2 the most frequently mutated gene of the five major PD genes.
Importantly, the high mutation frequency of the wild-type Cas9 plasmid was not merely due to homologous recombination of the plasmid with the genome.
Further exploratory analysis revealed that genes identified by two or more methods tended towards high mutation frequency (one-sided Wilcoxon P<7.8 × 10−13, Supplementary Fig. 4).
Furthermore, in the few collected seeds of SlIAA9-crispr mutants, a high mutation frequency was also detected (T1 seeds; Supplementary Fig. 6), and next generation plants showed the typical simple leaf phenotype upon germination (T1 seeds; Supplementary Fig. 6b).
Moreover, a high mutation frequency was strongly associated with the performance of all methods, particularly in terms of recall, regardless of whether that method had been specifically designed as a mutation frequency detector (Supplementary Figs 5 and 6).
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LeClerc, J. E., Li, B., Payne, W. L. & Cebula, T. A. High mutation frequencies among Escherichia coli and Salmonella pathogens.
Particular concerns arise for RNA viruses, which exhibit high mutation frequencies.
Two different concentrations of template DNA were used, according to the manufacturer protocol, for the generation of medium mutation frequencies (MMF) and high mutation frequencies (HMF).
This is the first report of high mutation frequencies of RASSF1 and RBSP3 genes in different epithelial malignancies.
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