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high mutation frequencies.
Particular concerns arise for RNA viruses, which exhibit high mutation frequencies.
Two different concentrations of template DNA were used, according to the manufacturer protocol, for the generation of medium mutation frequencies (MMF) and high mutation frequencies (HMF).
This is the first report of high mutation frequencies of RASSF1 and RBSP3 genes in different epithelial malignancies.
The high mutation frequencies generally observed in RNA virus populations are due to a fast rate of progeny production in the absence of proofreading mechanisms [2].
For example, strains of bacteria with high mutation frequencies can acquire resistance to antibiotics more rapidly than wild-type strains [33], [34], and also can exhibit enhanced horizontal acquisition of exogenous DNA [35].
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This high mutation frequency suggests that GNAQ mutational analysis can also be useful in the differential diagnosis of peripherally located melanotic schwannoma with blue nevus.
The high mutation frequency in both familial and sporadic patients makes LRRK2 the most frequently mutated gene of the five major PD genes.
"Finding this high mutation frequency is a breakthrough in the field of melanoma research," he says.
Therefore, two different PCR conditions to yield medium mutation frequency (MMF: 4 9 mut/kb) and high mutation frequency (HMF: 9 16 mut/kb) were adopted in this work, and a library of 5000 MMF and 5000 HMF mutants was generated.
In a previous work, we reported that P. aeruginosa has a high mutation frequency to fosfomycin resistance in vitro [24].
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