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c, d, Representative image of the PVN labelled with OT antibody (OT-Ab, magenta, left), OT-2A-Flp::FonGFP (OT-mRP, cyan, centre), and merged (right) at low magnification (scale bar, 200 μm) and high magnification (scale bar, 50 μm).
Matrices had abundant proximal airway-like structures that had multi-ciliated cells on the apical surfaced labeled by ACTTUB (red, top panel) in low (scale bar 50 µM) and high magnification (scale bar 10 µM).
Serial sections showed that cells were also FOXJ1 positive (white, lower panel) with the epithelium outlined in ECAD (green) in low (scale bar 50 µM) and high magnification (scale bar 10 µM).
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g, h, OT-Ab labelling (magenta, left) and Chronos-TdTomato expression (OT-vRp, cyan, middle) are co-localized (right) in the PVN at low magnification (c, scale bar, 200 µm) and high magnification (d, scale bar, 50 µm).
Representative whole mount images of spheroids in a matrigel droplet are shown at low (left, scale bar 200 µm) and high magnification (right, scale bar 100 µm).
Insets in (a) to (d) are at high magnification, and the scale bars are 2 μm.
(b) SAED patterns and (c) TEM images at high magnification (the inset scale bar is 10 nm) of the as-prepared hollow SnO2 nanoparticles, and (d) HRTEM image of a single SnO2 nanoparticle (the inset scale bar is 2 nm).
(b) SAED patterns and (c) TEM image at high magnification (the inset scale bar is 10 nm) of the as-prepared carbon-coated hollow SnO2 nanoparticles and (d) HRTEM image (d) of a single SnO2@C nanoparticle (the inset scale bar is 2 nm).
Bottom horizontal panel shows higher magnification images, scale bar = 10 μm.
Bottom horizontal panel shows higher magnification images, scale bar = 20 μm.
(The insets in a and b are the corresponding SEM images with high magnification and all the scale bars are 500 nm).
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