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The co-purification of GroEL1 with histidine-tagged recombinant proteins can be particularly problematic since native GroEL1 is expressed at relatively high levels, meaning that in the case of a low yield of recombinant protein, GroEL1 may well compete with the protein of interest for binding sites on nickel affinity beads.
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The UK's terror threat level remains at "severe", the second-highest level, meaning an attack is highly likely.
Britain has raised the threat level of an Irish-related terror attack from moderate to substantial, the third highest level, meaning a strike is a "strong possibility," the government said Friday.
Numbers remain at that new higher level, meaning civilian staff have had to take the hit.
The country's official terror threat level is at "severe" — the second-highest level — meaning an attack by extremists is considered highly likely.
In other management arenas, as well as in software engineering, configuration management has a high level meaning and concerns the arrangement of resources in an enterprise.
And the newest readings, announced on Tuesday, reveal the situation to be even worse than previously thought; the numbers have spiked by more than a fifth to their highest ever levels, meaning it can take just a few hours of exposure for the dosage to become lethal.
This is explained by the fact that the sensitive part is heavily suppressed at high-treatment levels, meaning that the dynamics are strongly influenced by the actual time point at which the first resistance mutation occurs.
Britain's current assessment of the threat of a terrorist attack stands at severe, its second-highest level, meaning that an attack is seen as "highly likely," according to the MI5 Web site.
A computer, though, is incapable of extracting that sort of high-level meaning; for it, the text is just long strings of ASCI characters.
In some specific cases, the level of concern had reached the second highest level meaning there had been "continued failure and/or failure to maintain an agreed improvement trajectory".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com