The high level of divergence, 21%, implied a substantial amount of multiple hits.
However, the genomic sequence within the 5' region of JV ORF1 (5'GS) displays a high level of divergence.
Exploring the genomes of the cellulolytic S. cellulosum (Sorangineae) and the bacteriolytic M. xanthus (Cystobacterineae) a high level of divergence was discovered [7].
In addition, we also noticed that for very high level of divergence (FST>0.4), estimates were strongly upwardly biased, especially in the case of model 1.
Such high sequence conservation for ORs from different species is an interesting feature considering that in general ORs display a high level of divergence [8].
The high level of divergence (5 11% from combined mtDNA loci) observed among C. megarhyncha lineages are similar to the levels separating different genera and even families of birds in North America [53], [54].
In order to accomplish this recognition function there should be at least one V1R receptor encoded by a gene exhibiting a high level of divergence, in the form of fixed nonsynonymous changes between the two subspecies arising by adaptive evolution.
For instance, comparing allele frequencies or differentiation among different breeds is straightforward to identify footprints of selection which are characterized by an unexpectedly high level of divergence, relatively to the neutral hypothesis [6], [7].
The high level of divergence between dipteran and lepidopteran apoLp-IIIs sequences may reflect the plasticity of apoLp-IIIs, as divergent amino acid sequences can fold into proteins with similar tertiary structures.
For the latter scenario, nevertheless, they together resulted in underestimation of differentiation at low level of pure-drift divergence (FST<0.05) while model 1 implementation became fairly inaccurate (e.g. Figure S2A) for high level of divergence (FST>0.2).
The level of sequence divergence amongst orthologous CPRs, for example, is often low outside of the conserved chitin-binding domain despite a high level of divergence among paralogues, indicating that these regions are functionally constrained.
