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One of the primer pairs and the corresponding probe displayed a high level of amplification efficiency on which a real-time RT-PCR method was established.
The L4417 template positive control was excluded from Fig. 2b because as expected it showed an extremely high level of amplification and PCR product.
Yet the CTCs obtained 34 and 24 months after diagnosis of the primary tumor and liver metastasis, respectively, revealed a high level of amplification of CDK8, which had not been noted in the previous analyses [ 37].
The high level of amplification is not compensated by a high turnover, i.e., the rapid disappearance of older lineages, as shown by the large diversity of mariner lineages and the high copy number in old lineages too.
Ninety-two cases had a high level of amplification (>6 copies per cell) by own CISH, 85 (92%) demonstrated some degree of HER2 gene amplification (≥6 copies per cell) by outside CISH.
Analysis of copy number abnormalities has shown a consistently high level of amplification of chromosomal region 8p11 containing the FGFR1 coding region in early-stage breast cancers, resulting in overexpression of FGFR1 [ 13].
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MM-BMMSCs displayed high levels of amplification of the backward region of C19MC.
Results: We show that the model for timing chromosomal amplifications is limited in scope, particularly for regions with high levels of amplification.
However, there always have concerns on whether high levels of amplification are necessary or if the gain of single extra copy would be able to advance cancer.
The detection of ultralow concentrations of nucleic acids such as microRNAs, pathogenic DNAs, and mutations in cancer requires high levels of amplification.
All seven IHC 2+ cases scored as having high levels of amplification by FISH were also scored as highly amplified (>6 copies) by CISH (100%) (Table 4).
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CEO of Professional Science Editing for Scientists @ prosciediting.com