The co-purification of GroEL1 with histidine-tagged recombinant proteins can be particularly problematic since native GroEL1 is expressed at relatively high levels, meaning that in the case of a low yield of recombinant protein, GroEL1 may well compete with the protein of interest for binding sites on nickel affinity beads.
The UK's terror threat level remains at "severe", the second-highest level, meaning an attack is highly likely.
Britain has raised the threat level of an Irish-related terror attack from moderate to substantial, the third highest level, meaning a strike is a "strong possibility," the government said Friday.
Britain's current assessment of the threat of a terrorist attack stands at severe, its second-highest level, meaning that an attack is seen as "highly likely," according to the MI5 Web site.
Numbers remain at that new higher level, meaning civilian staff have had to take the hit.
In some specific cases, the level of concern had reached the second highest level meaning there had been "continued failure and/or failure to maintain an agreed improvement trajectory".
The country's official terror threat level is at "severe" — the second-highest level — meaning an attack by extremists is considered highly likely.
A computer, though, is incapable of extracting that sort of high-level meaning; for it, the text is just long strings of ASCI characters.
No financing is available, however, and franchisees are expected to maintain a relatively high staffing level (meaning higher overhead costs).
More than half of the participants in each subgroup (except for Turkish women) had a middle to high educational level, meaning they had followed intermediate vocational training or higher.
