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Further observations show that if I is a good illumination image, the intensity of Ibright(x) is a very limited high intensity value and mostly tends to be 255.
As an example, a wild-type homozygous genotype at a particular SNP would have a high intensity value for the wild-type allelic probe, and little or no intensity for the alternative allelic probe.
These results suggest that there is a positive relationship between the microarray and RNA-seq data sets such that: (1) transcripts that yield a high intensity value on the microarray also yield a high read count based on the RNA-seq plandorm; and (2) transcripts that yield a low intensity value on the microarray also yield a low read count based on the RNA-seq platform.
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For example, with logarithmic enhancement, the low intensity pixel values can be enhanced at the loss of high intensity values [6]; with histogram equalization enhancement, the equalization may over-enhance the image, resulting in an undesired loss of visual data, quality, and intensity scale.
The resulting image is shown in Fig. 3 and has pixels with high intensity values where there are distinct changes of the intensities in the original image (Fig. 1).
An over-segmented object was wrongly split into two or more smaller objects due to high intensity values in inner-object regions when applying the edge-detection filter (see section on Automated Image Analysis).
High intensity values of lamin A were also found in intranuclear structures).
Note that high intensity values equal weak staining, and vice versa.
In most cases, regions with high intensity values of lamin A were surrounded by high curvature values.
The color corresponds to intensity values according to a so-called "jet map", i.e. blue and red elements represent low and high intensity values, respectively.
On the other hand, blocks of putative markers may result from more complex intensity patterns, e.g. when related prototypes show high intensity values for several "overlapping" conditions simultaneously.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com