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The homogenized solution was immediately subjected to high intensity probe sonication (Vibracell VCX130; Sonics, USA) for 5 min.
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First we evaluated if there was a tendency for highly correlating probes to also be high intensity probes.
The Affymetrix GeneChip® Mapping 500K Array, which contains a series of high intensity probes, is an efficient tool for accurately determining micro-alterations in the human genome [4], [15], [17], [18].
The use of a high-intensity probe sonication makes UAE an expeditious (7 min versus >1 h) and efficient (93.1 98.2% versus 87.5 94% of recovery) alternative as compared with extraction using an ultrasonic bath.
Inhibition of the proteasome under an apoptotic insult recovers the high intensity peak probing that the degradation of Cyt c in the cytoplasm is responsible of the low intensity peak.
In a porcine model we evaluated the safety and efficacy of a newly designed laparoscopic high intensity focused ultrasound probe and software that allows real-time ultrasound guidance during renal tissue ablation.
Genes were then ordered by an ad-hoc method (fitting the probe intensity histogram with a mixture of two gamma distributions, representing low and high intensities of probes, and computing the relative probability that transcripts are from the first or second distribution).
These specific probes had a higher percentage of probes with high intensity signals compared to non-perfect or no match probes: Choosing the 95%-quantile of the intensities of non-human control probes a higher fraction of gene- and transcript-specific probes showed intensities above this threshold than non-perfect or no-match probes (33.8%, 33.7%, 21.1%, 18.8%; respectively).
Thus a high intensity for a G-spot probe cannot be regarded as evidence that its target gene is highly expressed.
When one of these probes has a high intensity it is therefore likely that other G-spot probes have high intensities.
Both genders had high intensities of the HEX-labeled (CHD-ZW-common) probe, whereas only females had high intensity of the FAM-labeled (CHD-W-specific) probe.
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