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The expression level of plastocyanin gradually declined over the evaluation period, in contrast to the transient high induction at 1.0 and 3.0 hpi observed with flg22 Lso.
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SWT enhanced the levels of Nrf2 protein in a dose-dependent manner, with the highest induction at 2.56 mg/ml.
Although the Keap1 levels remained constant, oridonin enhanced the levels of Nrf2 protein in a dose-dependent manner, with the highest induction at 14 μM.
IRF-1 is also stimulated by rIL-1β but to a much reduced level, and interestingly it had the same expression profile as when stimulated with IFN-γ with highest induction at 24 h.
Six genes encoding putative antiviral and IFN-dependent genes from gene set 1 were selected due to high induction levels at this early time point.
For further clarification, sub-populations of the originally tested cell pools displaying high induction levels at low effector concentrations (30 ng/ml Dox) were enriched (>95%, Figure 5A).
At high induction of glnA, the abrupt stop is restored (Additional file 1: Figure S2).
In this validation set six genes showed a high induction after drug treatment in at least two of three CRC cell lines.
Although the model predicts well from low to medium range induction levels, our assumption was not valid at very high induction levels, in which lesser RFP expression was observed than predicted.
It is possible that at very high induction levels, the transcription and translation machinery in cells are fully saturated, thereby imposing metabolic burden on the cells and limiting protein production [ 39].
The response contour plots indicated that a combination of relatively high induction power and low deposition pressure at high bias voltage with low Ar dilution produces DLC films with high hardness.
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