TnsD directs high frequency orientation-specific insertion by TnsABC into a single site in the E. coli genome called attTn7 in a process that involves TnsD distorting the target site [33].
A simple and familiar color screen yields a high frequency of recombinants in both orientations.
The high frequency of Chi sites in both orientations in the genome (e.g., Figure S2) may be sufficient to create small symmetrical degradation and resynthesis tracts that cause the strong local hotspots at DSBs.
If the centrosome orientation mechanism is defective, a high frequency of centrosome misorientation would result, although this scenario does not necessarily lead to spindle misorientation.
This predisposition is also present in the maps generated by the model (Fig 7a), where dark (low frequency) and white (high frequency) regions tend to be align with regions of rapid orientation change.
However, the very high frequency of deletions observed in the CAG leading-strand template orientation was likely to be masking an elevated frequency of expansion (if the repeat array was deleted, it did not have the opportunity to expand).
Furthermore, the effect of orientation angles of laminations on SEA parameters are also discussed in mid and high frequency regions.
High frequency constant phase elements (CPEs) were exploited to determine oxide thicknesses as a function of crystal orientation.
While scoring centrosome orientation in GSCs expressing E-caddCR4h, we noticed that these GSCs had a high frequency of misoriented spindles during mitosis (∼39%, Figure 4A, C), while GSCs expressing E-cadDEFL did not (0%, Figure 4B, C).
In type I lissencephaly, EEG recordings, show high amplitude and high frequency discharges, due to the neocortical disorganization and smooth brain surface, where neurons are organized in similar orientations, leading to an amplified signal [40].
Such irregular metaphase-anaphase transition, before bi-orientation of all chromosomes, would readily explain our findings of unsynchronized sister-chromatid separation, a high frequency of non-disjunction, and diplochromosomes in metaphase spreads.
