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We defined a nuclear p53 signal that monotonically rose to and sustained at a high fluorescence level as 'monotonic increase'.
In a similar way, clones with high fluorescence level were selected as for high q in FITC-conjugated MTX-mediated gene amplification system on the basis of correlation of q with amplified gene copy number [ 7].
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High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase double deletion strain (gpd1Δgpd2Δ), which is deficient in the ability to regenerate NAD+ via glycerol formation.
Fluorescence fluctuations are shown in pseudo colors where high fluorescence levels appear yellow-green and low fluorescence levels appear blue.
In addition, high fluorescence levels were observed in MT1-MMP-expressing tumors in xenografted mice 24 and 48 h after MT1-hIC7L administration (Fig. 4).
Results from studies using a Syrian hamster cheek pouch tumour model (Andrejevic-Blant et al, 1997) demonstrated high fluorescence levels in endothelial cells at 8 h after Foscan, with accumulation in squamous epithelial tissues from 2 to 4 days.
The relative difference between ROS area with and without ABAP was considered a measure of antioxidant capacity, with high area difference meaning low antioxidant capacity, since high fluorescence levels were obtaining after adding ABAP, meaning low competence to neutralize peroxyl radicals.
Clinical studies in a group of patients treated with Foscan PDT for gynaecological cancers (Wierrani et al, 1997) or SCC of the upper airways (Andrejevic Blant et al, 2001) also demonstrated high fluorescence levels in endothelial and inflammatory cells during the first 20 h.
A significantly higher fluorescence level of the yellow fluorescent protein was observed when its encoded gene was integrated into the pCA2.4 native plasmid when compared to the isogenic chromosomally integrated control strain.
On average, a minimum of 20-fold higher fluorescence level could be achieved from integration into the native plasmid.
If >1 amplicon for a specific VNTR locus was detected and the size difference matched >1 repeat lengths (so-called stutter peaks), the one with the highest fluorescence level was used to calculate the repeat number.
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