We have implemented a combined high-throughput protein expression strategy with comprehensive profiling using an algal glycan-microarray that we call DB-CoMPP.
This group follows an expression strategy with very high TR and very low mRNA stability [7] that allows for fast changes in RA [5].
Though numerous strategy options are available for achieving high expression levels of genes in Escherichia coli, not every gene can be efficiently expressed in this organism.
Within the testing strategies, erlotinib was given to patients with high expression or a high copy number and docetaxel to those with low expression or a low number, respectively.
However, the strategy of controlling expression has a couple of limitations: the high expression of ALDH can be a burden on the host strain; the 3-HPA concentration cannot be decreased below a certain level which is dependent on the Km of ALDH for 3-HPA.
Groisillier, A. et al. MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms.
These observations suggest a promising strategy by targeting miRNAs to reverse gefitinib resistance in NSCLC with high expression of Axl [ 58, 68].
The secretory expression strategy of PelN proposed in this study achieved a relatively higher secretion capability.
Flow cytometric analysis of Jurkat-KI-R5 cells shows high expression levels of surface CCR5 in Jurkat-KI-R5 cells, indicating that the knock-in strategy works as expected to induce the expression of the endogenous CCR5 (Fig. 1E).
The present study applied the same strategy and did the analysis on the miRNAs with the following features: with high expression alteration and high expression level in at least one library.
