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The ALL xenograft mouse represents a high engraftment model.
While the derived masking approaches outlined in this study were used for a high engraftment model, this masking approach could be applied and adapted to other xenograft model systems where human tissue infiltration is below 90%.
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Therefore, without prior cell separation, profiling xenografts on whole transcript arrays is possible for high engraftment haematological models.
Despite high engraftment rates and attenuated disease progression (change in ejection fraction for EHMs, -6.7±1.4% versus control, -10.9±1.5%; n>12; P=0.05), we observed no difference between EHMs containing viable and nonviable human cardiomyocytes in this chronic xenotransplantation model (n>12; P=0.41).
Having the means in hand to delineate the transcriptional profile of human cells with a high engraftment potential provides an important instrument for future development in the field.
We observed a high engraftment rate of human CD45+ hematopoietic cells in all of these tissues.
High engraftment rates and low TRM suggest that ASCT is feasible and tolerable.
Here, we sought to validate species-specific gene expression profiling in the high engraftment continuous ALL NOD/SCID xenograft.
Comparison between 2D tissue culture and 3D organoid orthotopic engraftment models.
Comparatively, CD34 and total nucleated cell (TNC) were only weakly predictive in post-thaw neutrophil and platelet engraftment models, respectively.
Mouse engraftment models have been the standard assay used to identify blood stem cells.
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