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Polyelectrolytes typically have a very high effective concentration of charged groups and could constitute highly selective complexants.
The high affinity for Zn II) was mainly due to a high effective concentration of the fluorescent proteins and could be understood quantitatively by modeling the peptide linker between the fluorescent proteins as a random coil.
The formation of this crystallographically-established trans-fused acetal is discussed in terms of the high effective concentration of the intramolecular nucleophile which leads to a high degree of a SN2 character in the displacement of the α-glucosyl triflate or at the level of the corresponding α-CIP.
The advantage of this configuration is that reconstituted enzyme is exposed to a consistent and high effective concentration of substrate regardless of expression levels.
This signaling likely occurs through both high- and low-affinity receptors, due to the high effective concentration of locally produced ligand.
The high effective concentration of protein enabled EPR data collection with a high S/N ratio.
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With regards to its such high effective concentrations and its chemical family, this particular compound could be considered as a food attractant for Coquillettidia larvae [12].
Enzymes residing in the postsynaptic density that are involved in processes leading to potentiation and AMPA clustering, such as CaMKII, would be subject to high effective concentrations of calcium in response to synaptic stimulation.
This approach is parallel to many recent works that make use of microfluidic confinement to maintain high effective concentrations of target biomolecules and enable ultrasensitive detection.
The high effective concentrations (≈700 μM) that can be achieved without aggregation will provide high signal-to-noise data with these methods and enable real-time recording of transient changes initiated, for example, by pressure or temperature-jump methods designed to measure the kinetics of conformational exchange.
As well as providing drug delivery vehicles that will target particular cell types and deliver drugs on demand, there is the deeper understanding that will result from studying the interplay between intramembrane assembly and intermembrane adhesion, processes that require multivalent binding and the high effective concentrations produced in membranes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com