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Moreover, the nitrogen limitation was examined in parallel with high density stress to gain a better understanding of physiological responses specific to high density stress.
High density stress has been studied mostly from agronomic (e.g. yield) and physiological perspectives.
Molecular mechanisms underlying high density stress response have not been studied in great detail.
Drought can be one of many components of high density stress.
To gain better insight into the physiology of high density stress, differentially expressed genes associated with high density stress and not nitrogen limitation were analyzed from this point forward.
High density stress, also known as intraspecies competition, causes significant yield losses in a wide variety of crop plants.
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This suggests that high-density stress plays some role in promoting the process of masculinization.
In chemostat cultures with T. reesei, the most sensitive markers for process disturbances were the genes coding for hydrolytic enzymes (cbh1, egl1, bgl2, bga1), which were consistently down-regulated in response to various factors like high concentrations of polypropylene glycol, lack of aeration or high cell density stress.
However, high population density stress did not result in major detectable changes in the transcriptome at the early stages of barley and maize life cycles despite detectable morphological changes [ 13].
Global transcriptome and metabolic changes of rice grown under HD and/or LN growth conditions were examined to investigate major pathways involved in high population density stress response in rice.
The major phytohormone-responsive gene categories induced by high population density stress in Arabidopsis were related to benzothiadiazole (a salicylic acid analogue), abscisic acid and methyl jasmonate, while ethylene represented a lesser proportion of differentially regulated genes [ 14].
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