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The aim of this study was therefore to investigate the suitability of high density sphere (HDS) cultures for studies of cardiomyocyte-, endotheliand, and stem-cell biology.
First, the original contribution of K. Vukusic et al. describes the behaviour of cardiomyocytes, endothelial cells, fibroblasts, and c-kit-positive cardiac primitive cells in high density sphere cultures.
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In our previous study [ 18], we described a novel 3D model of high density spheres (HDS) cultured with or without cardiomyocytes.
The composite unit is a high-density sphere coated by soft silicon rubber.
We rapidly encapsulated iPSC-ECs at high density in poly ethylene glycol) (PEG) hydrogel spheres using thiol-ene chemistry and subsequently encapsulated cell-dense hydrogel spheres in a cell-free hydrogel layer.
Sphere formation was more efficient, when starting with a high density of cells: 1 × 10 cells/mL.
Two sets of meshes are generated for this case: 1) a high-density uniform mesh and 2) a mesh with higher density around the sphere boundary and source.
High density jellyfish meeting high density people".
Anything above 4g is deemed high density.
But what causes high density?
The high density of urban areas can be problematic.
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CEO of Professional Science Editing for Scientists @ prosciediting.com