Moreover, the excellent scalability of phase-change materials down to 2-nm size [112] also forecasts its potential to reproduce the ultra-high density neurons and synapses inside human brain.
High-density neuron cultures including dorsal root ganglia explants are employed for in vitro screening of biomaterials designed to control directional outgrowth.
Dendritic arbors of individual neurons in high-density neuron-glia co-cultures were visualized by expressing a MAP2B-EGFP construct under the control of the CAG promoter, which exhibits neuron-specific expression (Wayman et al. 2006).
To examine whether STIM1 is also localized on the surface of neurons, we surface biotinylated mature dissociated high density cortical neurons and examined the neutravidin precipitates via western blot.
V1 characteristically has a high density of neurons as a result of having smaller layer 4 neurons (von Economo 1929) and layer 3 pyramidal neurons (Elston and Rosa 1998).
The primate posterior medial OFC –the area damaged or disconnected in the patients– has a particularly high density of neurons that specifically fire when anticipated outcomes (rewards) fail to occur [20], [22].
Single cell recordings showed that this area contains a particularly high density of neurons that exclusively increase their discharge rate when anticipated reinforcements (rewards) fail to be delivered, that is, in trials whose repetition would induce extinction [20], [22].
However, given the high density of neurons and synapses in the vertebrate CNS, visualizing synapses in all cells at the same time makes it very difficult to discern connectivity patterns.
Due to the high density of neurons in the DG, the counting of total numbers of DGCs in the z-stack became very difficult (see DAPI image in Figure 3 figure supplement 1) and we thus used a compromise method to estimate DGC numbers by measuring the volumes of the DG region in the images.
In addition, CO blob regions respond to visual stimuli of lower spatial frequency than elsewhere in area 17 (Tootell et al. 1988b; Shoham et al. 1997) and have a high density of neurons preferring color stimuli (Livingstone and Hubel 1984a; Tootell et al. 1988a; Lu and Roe 2008).
Protein samples were prepared from high density hippocampal neuron cultures or murine neuroblastoma N2a.
