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Briefly, we investigated regions in 100 bp windows where the coverage in the germline was between 0.1 and 10 of the mode coverage in order to remove regions with high degrees of repeat sequence (for example, centromes or difficult to sequence regions.
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Intraspecific homogenization of the repeats could be explained by biased PCR amplification of a single repeat type in the repetitive region, however, our results are consistent with the high degree of repeat sequence conservation in AcSp1 sequences from araneids and L. hesperus[ 19].
The high degree of repeated sequence makes T. cruzi a suitable system for studying different kinds of repeat phenomena, such as gene conversion, chromosome rearrangements, and other evolutionary aspects.
Two asymptomatic DM1 individuals with relatively stable protomutations in blood (75 and 90 repeats) showed the highest degree of repeat size heterogeneity in sperm (even exceeding 1000 repeats) and the most dramatic allele size increase (on average 180 repeats in one individual and, even, 700 in another).
This high degree of repetition and inter-repeat similarity has undoubtedly hindered the assembly of full-length sequences from genomic DNA.
CL Brener has a relatively large genome when compared to other strains, which can in part be explained by the higher degree of repeated sequence [ 48].
In contrast to repeat units of type I they differ in length and orientation, contain inversions and show a high degree of interspersed repeats (55%).
Comparison of intergenic sequences to one another revealed numerous dispersed repeated sequences with either 100% or very high degrees of identity.
All guidelines achieved high degrees of consensus.
If polymerase slippage is the dominant force then we would expect to see a high degree of variability in repeat lengths and homogeneity between phylogenetic analysis carried out between sequence regions upstream and downstream of repeats [9], [35].
Messier et al. (1996) observed that once a tandem repeat expands above a threshold size (4 5 repeats for di- and 2 for tetranucleotide microsatellites, respectively), it exhibits a high degree of variation in repeat numbers among primate species.
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