Sentence examples for high coverage uniformity from inspiring English sources
Using a universal PCR protocol without optimization, these assays result in high coverage uniformity and limited non-specific coverage.
The use of individual qPCR reactions clearly resulted in a very high coverage uniformity.
In pursuit for finding viable solutions to the above-mentioned drawbacks and given that the development of scalable techniques [31 36] is one of the main concerns for the future of the emerging PV industry, here we present a modified scalable spray-coating technique to fabricate perovskite thin films with high coverage and uniformity.
Our results demonstrate that compared to mRNA-Seq and microarrays, Ribo-Zero-Seq provides equivalent rRNA removal efficiency, coverage uniformity, genome-based mapped reads, and consistently high quality quantification of transcripts.
The performance of the NGS technologies at low sequence coverage is correlated with per-base sequence coverage uniformity; where Roche 454, with the most uniform coverage, performs the best and suggests that for all the NGS technologies, achieving more uniform sequence coverage would result in considerably higher performance at lower coverage.
Given the 30 folds differences in genome size between C. elegans (~100 Mb) and human (~3,000 Mb) (Consortium, 1998; Venter, 2001), an NSR hexamer set generated for C. elegans had higher coverage and better uniformity read coverage than those applied in human.
Coverage uniformity is important so that one does not need to waist sequencing capacity for over-sequencing of some regions in order to obtain adequate coverage of others.
(see Supplementary Figure 1 for an illustration of coverage uniformity).
Coverage uniformity: The coefficient of variation was calculated for normalized mean coverages per exon.
None of the above referenced studies evaluate the coverage uniformity as a metric.
Therefore, it allows greater sequencing coverage uniformity and can identify also copy number variations, large insertions/deletions, and gene fusions.
