Beyond the slight gains by increasing the number of mismatches from 2 to 3 and from 3 to 4, the high coverage of SOLiD data practically eliminates any reference bias due to low mismatch numbers.
The high coverage of SGS data can also be utilized in downstream quantitative analysis.
However given the high coverage of NGS data, it is expected that most if not all non-repetitive regions should be present in the assembled contigs.
There is a high coverage of valid ethnicity data (92%).
Surveys are, therefore, not considered a valid source for the estimation of per capita consumption of alcohol (Stockwell et al., 2004), despite some recent recall methods achieving a high coverage of alcohol sales data (Stockwell et al., 2008).
The high coverage of Illumina sequence data also renders this method appropriate for multiplexing and SNP discovery and therefore a useful approach for landscape level studies of evolutionary ecology.
The high coverage of Illumina sequence data also renders this method appropriate for multiplexing and SNP discovery and therefore a useful approach for landscape level studies of evolutionary ecology, particularly given that Illumina sequencing is the most cost effective platform presently available [ 21].
Mitochondria may be expected to be especially common in tissues with high oxygen demands [ 30, 31], and the use of muscle tissue for genome sequencing could thus potentially explain the high coverage of mtDNA sequences in our data.> Our consensus mtDNA sequence from the wolverine differed from the previously published wolverine mtDNA sequence [ 32] by 23 SNPs and 12 indels.
Given the high coverage of the Casey Bergman's Illumina data (∼90x for the autosomes; ∼45× for the sex-chromosomes), its source (males from the same ISO1 strain used in the assembly), and the inherently low error rate of Illumina sequencing, genomic k-mers that are unmatched by the Illumina reads almost certainly are due to assembly errors (or to new mutations in the ISO1 strain).
The Italian breeds Casertana and Cinta Senese and the English breeds Tamworth and Gloucester Old Spots also had a high coverage of ROH 50-555% using NGS data).
