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The high correlation level between theoretically determined rupture forces and experimental data on binding energies for the large number of systems studied here implies that SMD is a promising tool for drug design.
We also find that, for other vertebrates, a high correlation level is not common, though most vertebrates tend to have higher positive correlations than other lower eukaryotes.
We validated this estimation by other two quantitative methods, confirming that only bird genomes can reach a high correlation level (0.95).
The visual symmetry in BSDT means high correlation level between AT and CG skews, and high visual symmetry in large scale appeared only in the BSDTs of bird chromosomes.
Pearson correlation between replicates was calculated for every gene in all the arrays, resulting in a very high correlation level, with a coefficient of >0.89 for every independent experiment in a pairwise comparison.
Pearson correlation between replicates was calculated for every gene in all the arrays, resulting in a very high correlation level, with a coefficient >0.95 for every independent experiment in a pairwise comparison.
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Meanwhile, graphical representation with 95% error bars (Fig. 4) showed that in the first subset (pairs including at least one arm muscle) correlation always crossed the 0 reference line (lack of correlation), while the second subset was consistently characterised by high correlation levels up to the most correlated pair, i.e EUC-OPP (untransformed r = 0.786).
The software programs showed high correlation levels for bandwidth, phase SD, and entropy (r ≥ 0.73, p < 0.001).
Similarly high correlation levels above r = 0.95 were observed in the remaining samples.
Moreover, our study highlights that high correlation levels are not necessarily associated with high agreement between raters.
In terms of relative bias, the WLW model shows the worst performance for low correlations but greatly improves for high correlation levels, independently of censoring and sample size.
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