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The wide range of WHIDE's applicability is demonstrated with examples from toponome imaging, high content screens and MALDI imaging (shown in the Supplementary Material).
The zebrafish is one of the most cost-effective vertebrates that can be used for high throughput and high content screens.
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Tsuji, N. et al. Whole organism high content screening identifies stimulators of pancreatic beta-cell proliferation.
Their activity was also evaluated against intracellular amastigotes by high content screening (HCS).
Cell-based high content screening (HCS) is becoming an important and increasingly favored approach in therapeutic drug discovery and functional genomics.
We have developed a novel in vitro assay using high content screening technology to measure changes in a number of cell physiological parameters simultaneously within a neuronal population.
This method observe living cells; images are acquired from microscopes and other high content screening systems.
These should also be cost effective and provide sufficient throughput for high content screening.
High content screening generates a more comprehensive cellular phenotype and therefore a more effective way to determine hits.
Fixed, stained cells were analyzed by ArrayScan®VTI High Content Screening (HSC) Reader (Cellomics, Pittsburgh, PA, USA) using Target Activation Bio Application (TABa).
This method could be scaled for use with higher throughput assaying via the FLIPR, or even for integration with high content screening platforms.
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