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Considering the high conformation similarities in contrast to huge differences of OPH activity between AbOPH, OPHC2 and MPH, a semi-rational design based on consensus sequence approach was performed to identify the key residues that depress the OPH activity of AbOPH.
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The data indicate that nascent proinsulin non-monomers shape quickly and have lower affinity with the high conformation-dependent insulin antisera [30] than with C-peptide antisera.
Of note, the non-natively folded proinsulin non-monomers showed lower affinity with the high conformation-dependent insulin antisera [30] than with C-peptide antisera (Figs. 2A, 2B, 3A, 3B).
Selection of animals with higher conformation, finishing precocity and muscling scores, especially at yearling, should promote an increase in cows' mature weight.
Higher conformation class and increased carcass weight in animals with disorders versus those without can probably be explained by the breed differences.
Sogstad et al. [ 50] found that lameness and lesions at the tarsus in dairy cattle were associated with lower conformation class and lower carcass weight, whereas sole ulcers were associated with higher conformation class.
Our findings demonstrate that an accurate balance between a rigid, high-affinity conformation and conformational flexibility is required to create an efficient and stringent molecular switch.
This evidence supports the above conclusion that ATP induces a conformational change in gp16 that causes it to assume a high affinity conformation for dsDNA binding.
As the majority of this reduced level of internalized LFA-1 is neither in extended nor high affinity conformation and detected with a pan-LFA-1 mAb, it must by default be in the bent, inactive conformation of LFA-1.
UV/VIS spectra of CYP11A1 displayed a pronounced peak at 392 nm, indicating that the protein is in its high spin conformation.
Depression has been linked to a selective increase in the high affinity conformation of the α2-adrenergic autoreceptors (α2-ARs) in the human brain as well as to an overexpression of α2-ARs in the hippocampus and cerebral cortex.
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