The decrease in R L with an increase in the initial concentration indicates that the adsorption is more favorable at high concentrations (figure not shown).
In the case of ECLDH, FBP and Mg2+ exhibited only slight activation effects on the enzyme reaction, and the latter even markedly inhibited the reaction at high concentrations (Figure 6, and Tables 2 and 3). Figure 6 Effects of intermediary metabolites and ions at pH 8.0.
Striking is the fact, that cholesterol strongly enhances the cytotoxic effects of BE but not BetA (Figure 1B and 1D) whilst being completely non-toxic on its own, even at very high concentrations (Figure 1E).
Antibodies to NTS-DBL1α domains from other PfEMP1 variants, which do not recognize R29 live infected erythrocytes by IFA, also did not induce phagocytosis of R29 infected erythrocytes, even at high concentrations (Figure 9, control PAR+, TM180, TM284, HB3R+).
In vitro, αIgM does not induce isotype switching to IgG3 whereas LPS is a mitogenic signal that does induce switching to IgG3, but at relatively high concentrations (Figure S2 and ([28]).
The Val187 variant of TAS2R9 showed a dramatic loss of function, with no responses to any of the bitter stimuli, even at high concentrations (Figure 2D, E and F and data not shown).
When the two U-runs were separated by 13 bases (2U-13), the size of SNF-RNA complex formed was still independent of the SNF/RNA ratio (Figure 2C, lanes 4 6), while SXL still formed a bigger complex with the same RNA at high concentrations (Figure 2C, lanes 1 3).
This toggle switch behaviour can be achieved for a very low concentration (figures 8A and 8B) and a high concentration (figures 8C and 8D).
Two of the compounds showed selectivity for Fas/FADD/Caspase-8 over Asc/Caspase-1, though displaying some inhibition of the latter in the yeast-based reporter gene assay at higher concentrations (Figures 6E, F).
