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Low and high concentration data were merged for data analysis.
Where Guinier plots revealed aggregation due to high concentration, data were removed (Guinier and Fournet, 1955).
(1) For identification of OP-specific gene changes, we removed the fenamiphos low concentration and the dichlorvos high concentration data from consideration.
In the SGSM 49-treated cells, we did observe a slight decrease in the level of NICD generation at a concentration of 30 μM; however, at this extremely high concentration, we also observed a decrease in holo-APP and β-actin levels that was supported by toxicity measurements at this very high concentration (data not shown).
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This decrease was not associated with cell death even at high concentrations (data not shown).
Digalloyl-resveratrol did not induce significant numbers of necrotic cells even at high concentrations (data not shown).
These values were further corrected to account for the quenching of these fluorophores that occurs at high concentrations (data shown in Figure S1 and methods provided in the Supporting Information).
Neither chlorpropamide (10, 30, 100 μM) nor gliclazide (10, 30, 100 μM) inhibited the ability of PDGF to stimulate [H]-thymidine incorporation even at high concentrations (data not shown).
For our investigation of proteins that change in abundance upon OP exposure, we combined the high concentrations data sets for dichlorvos and fenamiphos into one group and compared it to the combined unexposed controls for these exposures.
Whereas C1INH showed no significant binding even at the highest concentration (data not shown), specific protein interactions with CS-A were found for C1q, C4BP and factor H, thereby confirming the affinity chromatography results.
However, slight changes in morphology were observed for Vero cells at the highest concentration (data not shown).
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