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Kim, J. H. et al. High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
I dare say I gawped a bit: the dark brown eyes, the tumbling chestnut hair, the glowing skin, the generous mouth, the high cleavage across which she nervously tugs a skimpy cardie, the hourglass figure.
While no cleavage was observed on any of the targets by CjCas9 alone, high cleavage efficiencies with indels ranging from 25.5 to 37.9% in two targets and from 5.1 to 16.5% in one target were achieved when CjCas9 was assisted by SpdCas9 binding at various proximal locations (Fig. 5d).
This review will highlight the most recent progress in the area of artificial metallonucleases employing an oxidative mechanism, focusing on the development of methodologies for cleavage detection, understanding of the factors governing the cleavage efficacy and selectivity, and design strategies for the achievement of high cleavage efficacy.
The correlation between high cleavage fidelity and disruption of hydrogen bonds between the two domains leads us to propose that the high cleavage fidelity is the result of increased conformational flexibility of the mutant proteins.
It points out that this interface has a relatively low GB energy and correspondingly high cleavage energy.
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Good and fair quality oocytes produced a higher cleavage rate (P<0.01) than poor quality oocytes.
Our in vitro study using Rat-1 cells suggested higher cleavage specificity of CRISPR/Cas than that of TALENs against the albino mutation (Fig. 2b).
In particular, oocytes from group PBSCaMg free/FCS led to higher cleavage rates and blastocyst rate compared to the others.
Interestingly, the CRs group showed higher cleavage and blastocyst rates (76.8% and 29.6%, respectively) than the COCs (39.1% and 7.5%, respectively, P < 0.05).
Vitrified denuded oocytes matured with a co-culture system NkO-CC group, displayed higher cleavage rate and blastocyst production than vitrified COCs group.
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