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It applies a hierarchical screening procedure to identify molecular particles in EM micrographs.
All the above selection and screening methods establish fundamental conditions for the design of hierarchical screening projects.
This work presents how these different resources can be combined in a hierarchical screening strategy which has led to the identification of new A-site ligands.
Assays to be used in the early steps of a hierarchical screening programme demand well-characterised, immortal cell lines.
Large-scale screens for off-target activity within the kinome were done using the hierarchical screening protocol described previously.
This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system.
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This type of assay therefore has great potential as the first round in a hierarchical screen (Yarrow et al., 2005).
A key component of the proposed approach is an efficient and hierarchical computational screening that combines selection of materials with advanced process optimization.
The AutoDock4.01 program was used for the second step of the dual-step hierarchical virtual screening because of the better performance of its scoring function over those of the others for several target proteins[51].
To reduce computational cost while not degrade the calculation accuracy, two docking approaches, including Ligandfit and Autodock, were successively employed to dock candidates to the binding sites of these receptors in accordance with the order of their docking simulation accuracies in network-based dual-step hierarchical virtual screening.
To establish a hierarchical PCR screening system, the library was divided into 11 superpools each consisting of 12 plates of 384-wells.
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