Sentence examples for hey bud from inspiring English sources

"HEY bud, that will be $5," said the bouncer at the Whispers Nightclub in the Smithtown Sheraton, grabbing the arm of a dark-haired boyish-looking man who had just walked past him.

After 30 minutes he's like, 'Dude, you've got to tell him who you are.' So I said, 'Hey bud, by the way, I play for that minor league baseball team.' He stuck his foot in his mouth, and he was kind of quiet".

Mr. Abdomen will not show you a face pic, initially; that takes time, time that is filled with lots of vapid, two-word messages like "hey bud" and sharing photos of one another's genitals, or assumed genitals.

"I said, 'Hey bud, you can't support somebody underneath a... rock.' I said, 'To do your job at this level, at a place like this, you gotta be a grown... man to lead something.' I said, 'You can't lead anything under a... rock.' I said, 'You don't spend any time with us.

"Hey, bud," he gently croons.

"Hey, bud, what're you doing, huh?" he fondly inquires.

Hey budding rockstars, you hear that?

Western blot analysis showed that the expression levels of cyclin E and SKP2 were higher in HEY PGCCs with budding than in control HEY cells.

For ICC staining, HEY PGCCs with budding and control HEY cells were grown on glass coverslips until 70% confluence, washed with PBS, and fixed with cold 75% ethanol for 10 min on ice.

By using iTRAQ proteomic analysis and immunohistochemical staining, we found that HEY PGCCs with budding daughter cells abnormally express cell cycle-related proteins compared with diploid HEY cancer cells.

Based on iTRAQ proteomics analysis, western blot and immune staining, we confirmed that the expression of Cyclin E, SKP2, Stathmin in HEY PGCCs with budding daughter cells were higher than those in control HEY cells, which may provided new insight into how PGCCs and regular cancer cells are coordinately regulated in the progression of human ovarian carcinomas.