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In each age group, several follicles with early or advanced signs of atresia exhibited a heterogeneous staining pattern, which subsequently disappeared in late atretic follicles.
The 52 analysed tumour specimens revealed a heterogeneous staining pattern for IL-6 and TGF-β1 with variably intense cytoplasmatic and/or nuclear staining of the tumour cells.
Heterogeneous staining pattern was observed in the same well.
Most of the specimens displayed a heterogeneous staining pattern (data not shown).
Other tumour types that expressed CD70 (Table 2) also showed heterogeneous staining pattern with lower intensity.
CD34+ cells from JAK2 V617F positive patients (affected by ET, PV and PMF) displayed an heterogeneous staining pattern when probed with the JAK2 V617F /PNA.
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Apyrimidinic endonuclease-1 exhibits complex and heterogeneous staining patterns.
Rather heterogeneous staining patterns were observed within individual tumours, e.g. more pronounced infiltrations around necrotic areas.
Heterogeneous staining patterns were also present within metastases, and lymph node metastases from the primary tumour in some cases differed completely from each other.
In ALK fusion gene-positive samples stained with ALK iAEP IHC, almost all cancer cells stain positive, while heterogeneous staining patterns including a checker-board pattern indicated the expression of wild-type ALK [ 7, 8].
Moreover, there is still a large degree of uncertainty about the interpretation of complex and heterogeneous staining patterns in tissue samples and their relationship to the actual phosphorylation states in vivo.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com