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More powerful tests specially designed for this situation can find genes with heterogeneous expressions associated with possible subtypes of the cancer.
Previous reports have shown that the heterogeneous expressions of some genes like Nanog, Rex1 and Stella in ES populations are subject to epigenetic regulation and have equilibrium properties [26], [27], [28].
Although the heterogeneous expressions of these genes within neocortical areas have been reported in these previous studies, we think that more detailed analysis is necessary to understand their complex spatial distribution patterns.
Galectin-3 (Gal-3) and androgen receptor (AR) have been reported to play important roles in the progression of prostate cancer, and their heterogeneous expressions might be associated with different cancer subtypes.
Next, we analyzed a publicly available dataset from Affymetrix oligonucleotide arrays [data downloaded from Affymetrix (2009)], consisting of over 15 000 genes whose heterogeneous expressions comprising of human brain and heart cells were summarized using robust multi-array averaging (RMA) procedure (Irizarry et al., 2003).
We concluded that NY-ESO-1, LAGE-1, SCP-1 and SSX-4 genes may be promising candidates for cancer-specific immunotherapy in addition to MAGE, and that polyvalent cancer vaccines may be useful in cases of heterogeneous expressions of CTA genes in gastrointestinal and breast carcinomas.
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To investigate the heterogeneous expression of the newly identified broadly expressed RPC markers, section ISH and DISH were performed for one such gene, Fgf15.
Vector pET-28a for heterogeneous expression studies (Chen et al. 2015) was obtained from Novagen (Shanghai, China).
Heterogeneous expression of CD105 and CD73 was detected among SVF samples.
Thirty-five casexhibitedted a heterogeneous expression related in some instances to tumoral architecture.
Heterogeneous expression of EpCAM on cancer cells among different tissue or even within the same sample was observed [16, 19].
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