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It was thought that a limited vocabulary of forms would confer modern, machine-age unity upon the heterogeneous cultures of the world.
Transfected cells were exposed to increasing concentrations of HIV-1 clinical isolates from clades A, B, C, and F. Heterogeneous cultures of CD4 T cells expressing the U5 EGS (560) molecule were observed to maintain CD4 levels, were devoid of cytopathology, and did not produce HIV p24 gag antigen through 30 days after exposure to all HIV-1 clades at a multiplicity of infection of 0.01.
Moreover, differentiation of NSC often results in variable and heterogeneous cultures of neurons, glia and undifferentiated cells, which impedes many downstream applications requiring purified or defined cell populations, such as in vitro assays, transplantation and microarrays [12], [13].
Successful application examples in the literature focus on heterogeneous cultures of E. coli growth [ 34], yeast metabolic oscillations [ 35] as well as TRAIL-induced apoptosis [ 20].
Although bulk analysis of lineage-converted cultures suggested that these cells retained more residual gene expression from the starting somatic cells than iPSC-derived cells (Cahan et al, 2014), single-cell studies suggest that this reflects heterogeneous cultures of converted and non-converted cells rather than "confused" or mixed-property cells (Li et al, 2014).
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Further, the hNP cell system used here is highly purified and homogeneous, while rodent cultures are a heterogeneous culture of multiple cell types.
Use of this TH-RFP reporter cell line enabled purification of mDA-like neurons from heterogeneous cultures with subsequent characterization of neuron transcriptional and epigenetic programs (global binding profiles of H3K27ac, H3K4me1, and 5-hydroxymethylcytosine [5hmC]) at four different stages of development.
One possibility is that such compound conditions may select for propagation of heterogeneous cultures comprising cells co-habiting in different phases of pluripotency, as described for mouse EpiSCs (Bernemann et al., 2011; Tsakiridis et al., 2014).
When single HV low (HV−) or HV high (HV+) cells were cultured in 2i or 2i/LIF, they regenerated heterogeneous cultures, although a proportion of cells maintained a bias toward regenerating mixed populations that contained a higher fraction of the seeding cell type.
Only two instances of possibly heterogeneous cultures are observed.
In summary, NCAM immunopanning of hiPSC-derived heterogeneous cultures resulted in highly purified MAP2-positive immature deep-layer cortical neurons.
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