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After another 24 h, the suspension was carried to a hermetic bottle replenished with mineral medium to 100 ml, and 45 g m−3 of styrene was added.
A 90%H2O/10%% D2O solution (v/v) was prepared in a hermetic bottle to be used as eluent in the FIA system.
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The samples were then dried at 120°C, and finally kept in hermetic bottles for further experiments.
After inoculation with 1.7 L of pre-culture (obtained in 2-L hermetic bottles incubated for 24 hours at 30°C), the bioreactor had a final working volume of 17 L. Finally the pH was adjusted to either 7.3 or 5.2 ± 0.1 via automatic addition of sterile 1.5 N KOH combined with a Mettler Toledo probe (465-35-SC-P-K9/320) aneedlesles inserted through the septum.
It's not hermetic".
"Tully was just hermetic.
The hermetic secret.
Whipsawed by hermetic, aloof artworks?
Other works are more hermetic.
It's a little bit hermetic".
"Publishing is so hermetic sometimes".
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