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The following sense and antisense oligonucleotides were used, respectively: miR-126, 5′-CATTCTCGAGTGGCTGTTAGGCAT-3′ and 5′-TGGGCGGCCGCCTCTGCACTTCTT-3′ Amplified pre-miR-126 was subcloned into an empty pLemiR vector (Open Biosystems, Pittsburgh, PA, USA) and verified by sequencing, hereby referred to as pLemiR-126.
Hereby existential dependency needs to be verified (e.g. a disease is existentially dependent on the pathogen, but not vice versa).
Components appeared redundantly in different resources were excluded, and hereby a total of 369 A. oryzae genes (putative and experimentally verified) were included in our A. oryzae secretory component list, making it the most complete list so far for tracing the A. oryzae secretory machinery.
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In order to verify the experimental results, we hereby try to estimate the values of optical properties and the Brownian diffusion constant based on relevant data published by other researchers.
This is important, once studies, both in the Brazilian as well as in the foreign scenario have focused on correlational methodologies to verify the common points among the constructs hereby evaluated, which allows the establishment of a relation pattern between both, but not to specify the typical characteristics of the groups.
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