The sequence of 2,801 nt that contains no intron sequence and has the highest expression in flowers and leaves, was analyzed and annotated hereafter as cl1310.
Hereafter, all variants annotated in Exome Variant Server at the NHLBI (EVS, http://evs.gs.washington.edu/EVS/), dbSNP137 (http://www.ncbi.nlm.nih.gov/projects/SNP/), and Ensembl Gene Browser (http://www.ensembl.org) (MAF ≥ 0.01%) were excluded, and remaining variants confirmed by direct, bidirectional Sanger sequencing in two independent reactions.
All analysis hereafter refers to the annotated RefSeq genes.
On these grounds, we used the molecular function ontology (hereafter GO terms) to annotate each node of a PPI network.
> -wrap-foot> We then annotated protein-coding regions (hereafter referred to as "genes" unless otherwise indicated) from the transcriptome assembly.
We performed a series of BLAST searches to establish homology between contigs or singletons (both referred to hereafter as "ESTs") from Ae. albopictus and annotated genes from other organisms.
Using a stringent threshold (p < 0.005; see Methods) we found 6.8% (1,177) of coding RefSeq transcripts to be associated with long upstream antisense transcripts (hereafter, LUATs), of which 236 overlap with previously annotated non-coding transcripts.
Human BCRP [UniProt: Q9UNQ0] inhibition data was composed of data from the Open PHACTS Discovery Platform [3, 22] and of manually annotated data collected from literature sources [15], hereafter called pre-curated data.
In our analysis we annotated both contigs and singlets after assembly (hereafter referred as transcripts), since they contained different sequences and relevant information.
We trained TEES in a gold-standard set of 20 full text annotated documents, 10 on human embryonic stem cell research (hereafter called CF-hESC), whose entities annotations have been previously published (46) and a new set of 10 full texts documents on kidney stem cell research (hereafter called CF-Kidney).
