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Herds that had a different result in the bulk milk and seroprevalence studies were further investigated by whole herd analysis of individual serum samples to determine which classification was correct.
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The intra-herd analysis revealed more than one genotype in most herds with a similar distribution of clusters.
Even the intra-herd analysis confirmed that the types most frequently found were MVS 26 and MVS 27, while others types seem to be limited to some herds (e.g. type MVS 32).
For comparison of infected herds with control herds, univariable analysis using logistic regression was applied as an initial screening test to identify potential risk factors associated with the herds' infection status.
Herd composition analysis has been undertaken for households sampled for whom a percentage of the herd was away on transhumance (n = 26) and those for whom all cattle were in KGR (n = 23).
The serum samples were pooled by herd before analysis at SVA, with 10 individuals per pool.
The samples were pooled by herd, before analysis for the presence of C. burnetii DNA.
Herd level analysis of the risk factors identified an increase in herd size and ruminant composition as the major risk factors for herds to acquire Brucella infection.
Herd level analysis of the risk factors revealed that large and medium herds as well as herds kept with multiple livestock species were at higher risk of acquiring Brucella infection.
The similarity in results between cow and herd level analysis corresponds with our estimation of correlation between BTM antibody level and the within herd seroprevalence (R = 0.36; P < 0.001) in a previous study [ 6] of a subsample of 12 of the same herds as in the present study.
Based on results from the herd-level analysis showing that purchasing imported cattle was a significant risk factor for confirmed positive RHT herds, we would also expect that unconfirmed positive herds with a history of importing high risk cattle are less likely to be false positives than those without high risk imports.
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CEO of Professional Science Editing for Scientists @ prosciediting.com