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Unlike most other fragmentary jellyfish studies, we employed an integrative toxicological approach, including hemodynamics, clinical chemistry and hematology analyses, using N. nomurai jellyfish venom (NnV) in dogs.
These analyses more closely mirror the rapid blood chemistry and hematology analyses which often are used for near-patient testing and diagnosis.
C-reactive protein (CRP), estimated glomerular filtration tate (eGFR) and standard hematology analyses (hemoglobin, platelet, leukocyte and neutrophil counts) were measured at admission.
Blood was collected from all animals at 8 h and at 5, 9, and 12 d for toxicokinetic analyses (all time points) and for clinical chemistry and hematology analyses (days 5 and 12).
Hematology analyses were performed using an ADVIA 2120i (Siemens), and biochemistry analyses including C-reactive protein (CRP) were performed using an automated clinical chemistry analyzer (ADVIA 1800 Chemistry System, Siemens) following the manufacturer's instructions.
The CGPL offers two routine groups of hematology analyses for the GPs: "HEM": hemoglobin, mean red cell volume, red cell distribution width, total white blood cell count, and platelet count.
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Hematological analyses were performed on whole blood, using automatic counter (Mindray Auto hematology Analyser BC-5500) to evaluate following parameters: total white blood cells (TWBC), total lymphocytes using automatic Counter System (SB FACS) serologic parameters (CD8 and CD4) were determined [ 27].
Hematological analyses were performed using the ADVIA 120 hematology analyzer (Siemens, Deerfield, IL), and biochemical parameters with the Hitachi 917 chemistry analyzer (Roche Group, Tucson. AZ).
Hematological analyses (platelet count, red blood cell count, hematocrit values) were measured automatically using the hematology system ADVIA 120 (Siemens Healthcare Diagnostics, Eschborn, Germany).
The Cell-Dyn 3500 Hematology Analyser (Abbott Laboratories, Abbott Park, Illinois, USA) was used for the haematological analyses in 2006 and 2007.
For haematological analyses in 2006 and 2007, a Cell-Dyn 3500 Hematology Analyser (Abbott Laboratories, Abbott Park, Illinois, USA) was used.
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