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The lymphocyte and monocyte counts of peripheral blood from healthy controls and TB patients were analyzed using an automated hematologic analyzer XE-21000, Sysmex, Kobe, Japan) before plasma sample collection.
WT and PF4−/− mice were infected and on day 5 post infection brain mononuclear cells were isolated as we have described [10] and the total number of monocytes quantified using a mouse automated hematologic analyzer.
Total white blood cell counts, white blood cell differentials, red blood cell counts, platelet counts, hematocrit values, total hemoglobin, mean cell volume, mean corpuscular volume, and mean corpuscular hemoglobin concentration were determined from blood samples collected in vacutainer tubes containing EDTA, by using a laser-based hematologic Analyzer (Coulter Electronics, Hialeah, FL, USA).
The blood eosinophil counts were determined using an automatic hematologic analyzer.
An automated hematologic analyzer XE-21000, Sysmex, Kobe, Japan) was used to measure total and differential leukocyte counts.
Hematologic analysis was carried out using an automated hematologic analyzer (MS9 Differential Cell Counter 3 Part, HD Consortium, India).
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Platelets were analyzed by means of low-frequency detection with sheath flow technology using an automated hematologic analyzers (XE 2100®, Sysmex, Kobe, Japan).
Hematologic studies were performed by using an Advia 120 Hematology Analyzer (Bayer HealthCare, Diagnostics Division, Tarrytown, NY).
Extended Experimental Procedures Quantification of Hematologic Indices Hemoglobin, WBC, and platelet count per milliliter of blood were quantified on a Sysmex® KX-21N 3-part differential hematology analyzer.
Hematologic data including total white blood cells, total red blood cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, platelets, and lymphocytes were measured using a Coulter AcT 10 Hematology Analyzer (Beckman Coulter Inc., Miami, FL).
Eleven hematology analyzers were used.
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