Afterwards a string of journalists and communications helpers lined up to be greeted personally by the pope – some of the men embracing him in bear hugs.
Make sure you have a few helpers lined up for big jobs.
The vector was designed so that expression of a therapeutic gene is blocked in helper cell lines due to an intervening sequence containing various blocks in transcription and translation.
Mutant viruses lacking functional genes for gH (SCgHZ) [14], gB (HFEMdUL27-lacZ) [18] or gD (SC16gDdelZ) [18] were grown and assayed on helper cell lines expressing the corresponding glycoprotein: CR1 cells, expressing gH [19]; D6 cells expressing gB [13]; and VD60 cells expressing gD [18].
Because the response of various clones to 1,25(OH)2D3 may differ, we have examined the proliferative effects of the steroid on an antigen-specific cloned, nontransformed T helper cell line (D10.G4.1 [D10 cells]), and find that in contrast to these previous studies, the steroid is a potent stimulator of lectin-induced proliferation.
Horowitz, Jay Bruce, "Autocrine growth regulation of a cloned murine T helper cell line" (1987).
Each of the mutant neo constructs was introduced into the packaging helper cell line psi 2 by sequential cotransfection, and individual psi 2 double transformants were selected.
Purified preparations were assayed for particle numbers using electron microscopy [21] and for infectivity by plaque assay on Vero cells or, in the case of glycoprotein-deficient virions, on the relevant helper cell line.
To confirm the inhibitory role of MS4a4B in T cell propagation, we used synthesized siRNAs to silence expression of MS4a4B in T32 cells, a T helper cell line derived from normal primary T cells, which normally expresses high levels of MS4a4B protein [1].
We use two helper functions, decrypt_data (lines 67-81) and encrypt_data (lines 83-97) to keep the $documentt).ready functions neater.
